SUN Probes from IDT Demonstrate Superior Results to VIC Probes in qPCR
This technical note talks about the role of Fluorophore/Dye in a polymerase chain reaction (PCR). The fluorophore in absolute PCR quantification acts like a label that helps measure the amount of deoxyribonucleic acid (DNA) being amplified. It attaches to the DNA during the PCR process, making it easier for scientists to see and measure how much DNA is present in the sample. This helps when accurately determining the initial amount of DNA in the starting material.
A better-performing dye in PCR can improve the efficiency of the process by enhancing the detection of the DNA being amplified. When the fluorophore works more effectively, it produces stronger signals that are easier to detect and measure accurately. This clearer signal helps in better tracking and quantifying the DNA throughout the PCR process, leading to more precise and reliable results. Ultimately, a superior fluorophore can contribute to increased sensitivity and accuracy in PCR, improving its overall efficiency.