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TNF-alpha (TNF-a) Plasma

Test Code: 1230
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Clinical and Procedure
Clinical Utility

Cytokines have emerged as molecules of importance in the regulation of many immunologic processes in the cell. The ability to accurately measure quantitative and qualitative differences in cytokine production is becoming increasingly important to the understanding of normal and pathological processes.


Multiplex array format with Meso Scale Discovery (MSDĀ®) Sector Imager 2400. MSD Cytokine assays measure from one to ten cytokines in a 96 well MULTI-SPOT plate. The assay employs a sandwich immunoassay format. MSD technology uses electrochemiluminescence detection; a CCD camera allows for the quantification of light emitted from each spot in each well. MSD software generates a standard curve to determine sample cytokine concentrations. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.

Turnaround Time

3-5 business days from receipt of specimen

Specimen Information
Specimen Type Test Code CPT Code NY Approved Volume Assay Range Special Instructions
plasma 1230 83520 Yes

1 mL (min. 100 uL)

1.5 - 1500 pg/mL

  • 1 mL, plasma, ship frozen overnight on dry ice.
  • Stability:14 days frozen, stable 3 freeze/thaw cycles.

The reference range for a healthy population is less than 4.7 pg/mL. However it should be noted that these ranges are obtained from a limited population of apparently healthy adults and are not diagnostic thresholds.


Specimens are approved for testing in New York only when indicated in the Specimen Information field above.

The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for general informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.


Chung KF Cytokines: An overview in New Drugs for Asthma, Allergy and COPD, Prog Respir Res. 2001, vol 31: 242-246.

MSD 96-Well MULTI-ARRAY and MULTI-SPOT Human Cytokine Assays: Ultra Sensitive Kit package insert. Meso Scale Discovery

Bienvenu JA et al. Cytokine assays in human sera and tissues. Toxicology 1998; 129: 55-61.

Debad, J.D., Glezer, E.N., Wohlstadfer, J.N., Sigal, G.B. (2004) Clinical and Biological Applications of ECL. In Electrogenerated Chemiluminescence, ed. A.J. Bard. Marcel Dekker, New York, pp. 43-78.

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