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Innovations in Immunity

The ExPeCT anti-CD19 CAR T assay is an intervention tool which allows the Clinical team to provide patient care by directly monitoring the effectiveness of CAR T-cell therapy

  • Assess current immune response

  • Expansion and persistence of CAR T-cells

  • Evaluate treatment decisions

Learn more about Eurofins Viracor ExPeCT

Patient getting CAR T cell therapy infusion
ExPeCT anti-CD19 (FMC63) CAR T

ExPeCT anti-CD19 (FMC63) CAR T qPCR will allow you to personalize patient care by directly following the expansion and persistence of CAR-T cell therapy. 

Longitudinally monitoring CAR-T expansion / persistence through our qPCR assay gives you real-time data of the CAR-T therapy. 

This qPCR is meant to be an adjunctive measure to your current protocol providing further clinical insights into your patients. 


personalized patient care

The Advantages of ExPeCT anti-CD19 (FMC63) CAR T

Michelle Altrich quote for CAR T-cell therapy

CAR T infusion bag - Photo by Penn Medical

For Clinical Staff

  • Provides interventional data for therapeutic treatment
  • Quantifiable, numerical values for accurate measurement
  • Ability to longitudinally monitor status of CAR T-cells
CAR T Lab technician

For Lab Staff

  • Collection does not require additional staff or training
  • Simple blood draw, no extra processing to isolate PBMC
  • Not a "live cell" assay


The Eurofins Viracor ExPeCT™ (Expansion and Persistence of CAR T-cells) anti-CD19 (FMC63) CAR T

CAR T Pediatric Patient

When it comes to CAR T-cell therapy for pediatric patients, several factors need to be considered during laboratory testing to ensure optimal treatment outcomes


These considerations include;

  • the unique biological characteristics of pediatric patients
  • the specific types of cancers prevalent in this population
  • the potential impact of prior treatments


interventional data providing a reliable means of longitudinal monitoring anti-CD19 CAR-T cell expansion and/or persistence

How It Works

ExPeCT anti-CD19 (FMC63) CAR T
Eurofins Viracor has developed and validated a multiplexed quantitative real-time PCR (qPCR) assay targeting FMC63 for the longitudinal monitoring of CD19-directed CAR T-cell therapy.


How CAR T-cells are manufactured

CAR T process diagram infographic
CAR T abstract poster

Development of a Multiplexed Quantitative PCR Assay for CD19-Directed CAR T-Cell Persistence Monitoring

Chimeric antigen receptor (CAR) T-cell therapy has transformed hematological cancer treatment, with six therapies now approved by the FDA. Four of these therapies (tisa cel, axi cel, brexu cel, and liso cel) target CD19 using single chain variable fragments (scFvs) derived from the heavy and light chain variable (V H and V L ) regions of the monoclonal antibody FMC63.

The primary objective of this study was to evaluate the ability of FMC63 scFv specific real time PCR (qPCR) to detect and quantify FMC63 DNA in whole blood specimens. This assay is intended for the quantitative detection of FMC63 DNA from CAR T-cells in whole blood specimens for the purpose of monitoring CAR T-cell engraftment, expansion, and persistence in individuals receiving CD19 directed CAR T-cell therapy.

Here we describe the development, validation and performance characteristics of a multiplexed, quantitative qPCR assay targeting FMC63 for the monitoring of CD19 directed CAR T-cell therapy.

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Listen to Our Latest CAR T Podcast

For Hematology/Oncology patients the ExPeCT anti-CD19 (FMC63) CAR T assay provides interventional data which may provide information on the expansion and persistence of the genetically modified T-cells used as therapeutic treatment for specific lymphomas and leukemias (Chimeric Antigen Receptor T-cell therapy or CAR T). Eurofins Viracor has developed and validated a multiplexed quantitative real-time PCR (qPCR) assay targeting FMC63 for the longitudinal monitoring of CD19-directed CAR T-cell therapy.


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