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5000 - Atypical Pneumonia Panel - 3/28/2024 1:29:54 PM
Page of
Atypical Pneumonia Real-time PCR Panel
Test Code: 5000
Clinical and Procedure
Clinical Utility
Chlamydophila pneumoniae
is a common cause of pneumonia throughout the world and causes 7% to 10% of community-acquired pneumonia among adults. Symptoms of infection with
C. pneumoniae
are indistinguishable from other causes of pneumonia. A physical examination or chest x-ray does not typically provide information which allows for a definite diagnosis. Culture of the organism is technically demanding and time-consuming. Alternatively, molecular assays such as PCR allow for rapid testing of respiratory secretions for the presence
C. pneumoniae
DNA, thereby allowing a diagnosis to be established and therapy instituted quickly.
About Atypical Pneumonia
Mycoplasma pneumoniae
is the pathogen most often associated with atypical pneumonia. Often indistinguishable from other viral and atypical bacterial pathogens,
M. pneumoniae
causes a wide range of respiratory infections, including pneumonia, tracheobronchitis, and upper respiratory tract infection. Specialized laboratory tests are necessary to establish a definitive diagnosis due to the nonspecificity of atypical pneumonia clinical presentation. Traditionally, culture and serological tests have been utilized. However, culture is relatively insensitive for acute diagnosis and requires a long incubation period. PCR technology to assess for
M. pneumoniae
nucleic acid has proven to be a sensitive and specific diagnostic tool. Respiratory specimens are tested for the presence
M. pneumoniae
DNA, facilitating a rapid diagnosis which allows appropriate antimicrobial therapy to be instituted quickly.
Procedure
Extraction of
C. pneumoniae
DNA and
M. pneumoniae
DNA from specimen followed by amplification and detection of
C. pneumoniae
DNA and
M. pneumoniae
DNA using real-time, qualitative PCR. An internal control is added to ensure the extraction was performed correctly and the PCR reaction was not inhibited. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.
Specificity
The
Chlamydophila pneumoniae
PCR assay was tested for cross reactivity against
Bordetella pertussis
,
Bordetella parapertussis
,
Bordetella bronchiseptica
, all relevant non-
C. pneumoniae
species of
Chlamydophila
, all relevant strains of
Mycoplasma pneumoniae
, and all relevant species of
Legionella
as well as human herpes viruses, polyoma viruses, hepatitis viruses, adenoviruses, parvovirus B19,
Pneumocystis jirovecii
and
Toxoplasma gondii
with no cross reactivity noted.
The
Mycoplasma pneumoniae
PCR assay was tested for cross reactivity against
Bordetella pertussis
,
Bordetella parapertussis
,
Bordetella bronchiseptica
, all relevant species of
Chlamydophila
, all relevant species of
Legionella
as well as human herpes viruses, polyoma viruses, hepatitis viruses, adenoviruses, parvovirus B19,
Pneumocystis jirovecii
and
Toxoplasma gondii
with no cross reactivity noted.
Turnaround Time
Same day (within 8-12 hours from receipt of specimen), Monday through Saturday.
Specimen Information
Specimen TypeOrder CodeCPT CodeNY ApprovedVolumeAssay RangeBAL500987486 (x1), 87581 (x1)Yes
2 mL (min. 0.5 mL)
Detected/Not Detected
Special Instructions
-
Collect in a sterile, screw top tube.
-
Can be shipped at ambient or frozen temperature Monday through Friday.
-
Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
nasal wash501387486 (x1), 87581 (x1)Yes
2 mL (min. 0.5 mL)
Detected/Not Detected
Special Instructions
-
Collect in a sterile, screw top tube.
-
Can be shipped at ambient or frozen temperature Monday through Friday.
-
Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
trach asp501987486 (x1), 87581 (x1)Yes
2 mL (min. 0.5 mL)
Detected/Not Detected
Special Instructions
-
Collect in a sterile, screw top tube.
-
Can be shipped at ambient or frozen temperature Monday through Friday.
-
Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
throat gargle502387486 (x1), 87581 (x1)Yes
2 mL (min. 0.5 mL)
Detected/Not Detected
Special Instructions
-
Collect in a sterile, screw top tube.
-
Can be shipped at ambient or frozen temperature Monday through Friday.
-
Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
NP aspirate502487486 (x1), 87581 (x1)Yes
2 mL (min. 0.5 mL)
Detected/Not Detected
Special Instructions
-
Collect in a sterile, screw top tube.
-
Can be shipped at ambient or frozen temperature Monday through Friday.
-
Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
NP swab502587486 (x1), 87581 (x1)Yes
2 mL
Detected/Not Detected
Special Instructions
-
Place sterile swab placed in 2 mL sterile saline, M4, or viral transport media in a sterile, screw top tube.
-
Do not use calcium alginate swab or wood shafted swab.
-
Can be shipped at ambient or frozen temperature Monday through Friday.
-
Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
bronch wash502687486 (x1), 87581 (x1)Yes
2 mL (min. 0.5 mL)
Detected/Not Detected
Special Instructions
-
Collect in a sterile, screw top tube.
-
Can be shipped at ambient or frozen temperature Monday through Friday.
-
Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
nasal swab503087486 (x1), 87581 (x1)Yes
2 mL
Detected/Not Detected
Special Instructions
-
Place sterile swab placed in 2 mL sterile saline, M4, or viral transport media in a sterile, screw top tube.
-
Do not use calcium alginate swab or wood shafted swab.
-
Can be shipped at ambient or frozen temperature Monday through Friday.
-
Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
nasal asp503187486 (x1), 87581 (x1)Yes
2 mL (min. 0.5 mL)
Detected/Not Detected
Special Instructions
-
Collect in a sterile, screw top tube.
-
Can be shipped at ambient or frozen temperature Monday through Friday.
-
Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
NP wash504787486 (x1), 87581 (x1)Yes
2 mL (min. 0.5 mL)
Detected/Not Detected
Special Instructions
-
Collect in a sterile, screw top tube.
-
Can be shipped at ambient or frozen temperature Monday through Friday.
-
Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
trach wash504887486 (x1), 87581 (x1)Yes
2 mL (min. 0.5 mL)
Detected/Not Detected
Special Instructions
-
Collect in a sterile, screw top tube.
-
Can be shipped at ambient or frozen temperature Monday through Friday.
-
Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
Shipping
Ship Monday through Friday. Friday shipments must be labeled for Saturday delivery. All specimens must be labeled with patient's name and collection date. A Viracor Eurofins test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: Viracor Eurofins, 18000 W 99th St. Ste, #10, Lenexa, KS 66219
Causes for Rejection
Wood shafted swab, calcium alginate swab, specimens received in trap containers, specimens beyond their acceptable length of time from collection as listed in the specimen handling, or specimen types other than those listed.
Disclaimer
Specimens are approved for testing in New York only when indicated in the Specimen Information field above.
The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.
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